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VGLUT2 Recombinant antibody, PBS Only (Detector)

VGLUT2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241255B5

Cat no : 84083-2-PBS

Synonyms

SLC17A6, DNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, 241255B5

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using VGLUT2 Recombinant antibody (84083-2-RR)

    WB analysis of mouse brain using 84083-2-RR (same clone as 84083-2-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 84083-2-RR (VGLUT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84083-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1

    Cytometric bead array standard curve of MP00998-1, SLC17A6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84083-1-PBS. Detection antibody: 84083-2-PBS. Standard: Ag29565. Range: 0.313-40 ng/mL.

Product Information

84083-2-PBS targets VGLUT2 as part of a matched antibody pair:

MP00998-1: 84083-1-PBS capture and 84083-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen VGLUT2 fusion protein Ag29565
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Calculated Molecular Weight 64 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberNM_020346
Gene Symbol VGLUT2
Gene ID (NCBI) 57084
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicles (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).