SLC16A11 Recombinant antibody

SLC16A11 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240081H5

Cat no : 83239-5-RR

Synonyms

Monocarboxylate transporter 11, MCT11, MCT 11, FLJ90193, 240081H5

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Validation Data Gallery

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  WB analysis using 83239-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83239-5-RR (SLC16A11 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  FC experiment of A549 using 83239-5-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug SLC16A11 Recombinant antibody (83239-5-RR, Clone:240081H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83239-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83239-5-RR against Human SLC16A11 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse brain tissue, mouse stomach tissue
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83239-5-RR is SLC16A11 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC16A11 fusion protein Ag24920
• Full Name: solute carrier family 16, member 11 (monocarboxylic acid transporter 11)
• Calculated Molecular Weight: 471 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC093860
• Gene Symbol: SLC16A11
• Gene ID (NCBI): 162515
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.