SLAM/CD150 Monoclonal antibody

SLAM/CD150 Monoclonal Antibody for WB, ELISA

Host / Isotype

mouse / IgM

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5B4G1

Cat no : 60043-1-Ig

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

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Validation Data Gallery

• 

  WB analysis of Raji using 60043-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60043-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60043-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, HUVEC cells, Jurkat cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

60043-1-Ig targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: monkey
• Host / Isotype: mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLAM/CD150 fusion protein Ag1386
• Full Name: signaling lymphocytic activation molecule family member 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC012602
• Gene Symbol: CD150
• Gene ID (NCBI): 6504
• RRID: AB_2187821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

Publications

Species	Application	Title
monkey	WB	Animals (Basel); Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication; Authors - Haofei Song ; KD Validated