SLAM/CD150 Monoclonal antibody, PBS Only

SLAM/CD150 Monoclonal Antibody for WB, ELISA

Host / Isotype

mouse / IgM

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5B4G1

Cat no : 60043-1-PBS

Synonyms

CD150, CDw150, IPO 3, SLAM, SLAMF1

---

Validation Data Gallery

• 

  WB analysis of Raji using 60043-1-Ig (same clone as 60043-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60043-1-Ig (same clone as 60043-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HUVEC using 60043-1-Ig (same clone as 60043-1-PBS)

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60043-1-PBS in a different storage buffer formulation.

---

Product Information

60043-1-PBS targets SLAM/CD150 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLAM/CD150 fusion protein Ag1386
• Full Name: signaling lymphocytic activation molecule family member 1
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC012602
• Gene Symbol: CD150
• Gene ID (NCBI): 6504
• RRID: AB_2187821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Thiophilic affinity chromatograph
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.