SIX1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SIX1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10709-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of A2780 using 10709-1-AP (same clone as 10709-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 10709-1-AP (same clone as 10709-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SKOV-3 using 10709-1-AP (same clone as 10709-1-PBS)

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10709-1-AP (same clone as 10709-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10709-1-AP (same clone as 10709-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10709-1-AP (same clone as 10709-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10709-1-AP (same clone as 10709-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 10709-1-AP (same clone as 10709-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10709-1-AP (SIX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 10709-1-AP (same clone as 10709-1-PBS)

  IP result of anti-SIX1 (IP:10709-1-AP, 3ug; Detection:10709-1-AP 1:1000) with HEK-293 cells lysate 2560ug. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

• 

  IF Staining of epithelial tumor cells using 10709-1-AP (same clone as 10709-1-PBS)

  IF result of anti-SIX1 (1:50) in p53-/- mammary epithelial tumor cells. Blue: hoechst nuclear stain, Red: SIX1 stain the mesenchymal cell which have undergone EMT. This data was developed using the same antibody clone with 10709-1-PBS in a different storage buffer formulation.

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Product Information

10709-1-PBS targets SIX1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIX1 fusion protein Ag1044
• Full Name: SIX homeobox 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-35 kDa
• GenBank Accession Number: BC008874
• Gene Symbol: SIX1
• Gene ID (NCBI): 6495
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.