SIRT4 Monoclonal antibody, PBS Only

• 

  WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 66543-1-Ig (same clone as 66543-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NCCIT using 66543-1-Ig (same clone as 66543-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 66543-1-Ig (same clone as 66543-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SIRT4 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 66543-1-PBS

Clone No.1D5A2

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

SIR2 like protein 4, SIR2L4, SIRT4

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Product Information

66543-1-PBS targets SIRT4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT4 fusion protein Ag17347
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC109319
• Gene Symbol: SIRT4
• Gene ID (NCBI): 23409
• RRID: AB_2881905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6E7
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.