SIRT4 Monoclonal antibody

• 

  WB analysis of HeLa using 66543-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66543-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66543-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66543-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

SIRT4 Monoclonal Antibody for WB, ELISA

Cat No. 66543-1-Ig

Clone No.1D5A2

Publications(23)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, ELISA

Synonyms

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, NAD-dependent ADP-ribosyltransferase sirtuin-4, NAD-dependent protein biotinylase sirtuin-4

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cell, PC-3 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:80000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66543-1-Ig targets SIRT4 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT4 fusion protein Ag17347
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Calculated Molecular Weight: 314 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC109319
• Gene Symbol: SIRT4
• Gene ID (NCBI): 23409
• RRID: AB_2881905
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y6E7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB,IF	ACS Nano; Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer; Authors - Lingtong Meng
human	WB	Cell Death Differ; SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.; Authors - Shuai Leng ; KD Validated
human	WB	Cancer Lett; SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.; Authors - Mengqi Liu
	WB	iScience; Sirt6 regulates the proliferation of neural precursor cells and cortical neurogenesis in mice; Authors - Yufei Wei
human	WB	Int Immunopharmacol; Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma; Authors - Jiayuan Liang
human	WB,IHC,IF	Lab Invest; Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss; Authors - Yi Yi