SIRT4 Monoclonal antibody

• Featured Product
• KD/KO Validated

SIRT4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

1D5A2

Cat no : 66543-1-Ig

Synonyms

SIR2 like protein 4, SIR2L4, SIRT4

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Validation Data Gallery

• 

  WB analysis of HeLa using 66543-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 66543-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCCIT using 66543-1-Ig

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 66543-1-Ig

  L02 cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, NCCIT cell, PC-3 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:80000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

66543-1-Ig targets SIRT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT4 fusion protein Ag17347
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
• Calculated molecular weight: 314 aa, 35 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC109319
• Gene symbol: SIRT4
• Gene ID (NCBI): 23409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Publications

Species	Application	Title
mouse,rat	WB,IF	ACS Appl Mater Interfaces; Novel Thermosensitive Hydrogel Promotes Spinal Cord Repair by Regulating Mitochondrial Function.; Authors - Yi Li
human	WB	Cancer Med; PMID: 31573734; Authors - Jilin Xing ; KD Validated
human	WB	J Cancer; miR-424-5p regulates cell proliferation and migration of esophageal squamous cell carcinoma by targeting SIRT4.; Authors - Ying Cui
rat	WB,IHC,IF	Int J Biochem Cell Biol; SIRT4 regulates rat dental papilla cell differentiation by promoting mitochondrial functions.; Authors - Haoling Chen ; KD Validated
human	WB	Sci Rep; Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.; Authors - Kalaiselvi Sivalingam
human,mouse	WB	Front Pharmacol; Rosmarinic Acid Inhibits Mitochondrial Damage by Alleviating Unfolded Protein Response.; Authors - Guoen Cai