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  • KD/KO Validated

SIRT4 Monoclonal antibody

SIRT4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A2

Cat no : 66543-1-Ig

Synonyms

SIR2 like protein 4, SIR2L4, SIRT4



Tested Applications

Positive WB detected inHeLa cells, NCCIT cell, PC-3 cells, L02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:80000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66543-1-Ig targets SIRT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SIRT4 fusion protein Ag17347
Full Name sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC109319
Gene Symbol SIRT4
Gene ID (NCBI) 23409
RRIDAB_2881905
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.

Protocols

Product Specific Protocols
WB protocol for SIRT4 antibody 66543-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.

Authors - Shuai Leng
  • KD Validated
humanWB

Cancer Lett

SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer.

Authors - Mengqi Liu
humanWB

Int Immunopharmacol

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma

Authors - Jiayuan Liang
humanWB,IHC,IF

Lab Invest

Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss

Authors - Yi Yi
humanWB

Dis Model Mech

Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner.

Authors - Leonie Drews
humanWB

Sci Rep

Influence of psychostimulants and opioids on epigenetic modification of class III histone deacetylase (HDAC)-sirtuins in glial cells.

Authors - Kalaiselvi Sivalingam