CoraLite® Plus 488-conjugated SIRT2 Monoclonal antibody

SIRT2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8G10

Cat no : CL488-66410

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66410

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66410 (SIRT2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66410 targets SIRT2 in IF applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT2 fusion protein Ag7756
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC003547
• Gene Symbol: SIRT2
• Gene ID (NCBI): 22933
• RRID: AB_2883329
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The silent information regulator(SIR2) family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed, highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. SIRT2 exists various isoforms and the molecular weight of isoforms are 35kDa, 40 kDa, and 42 kDa.