CoraLite® Plus 488-conjugated SHMT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SHMT1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-30192

Synonyms

EC:2.1.2.1, CSHMT

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-30192

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 SHMT1 antibody (CL488-30192) at dilution of 1:200, CL594-Phalloidin (red).

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  FC experiment of HeLa using CL488-30192

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated SHMT1 Polyclonal antibody (CL488-30192)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30192 targets SHMT1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHMT1 fusion protein Ag31269
• Full Name: serine hydroxymethyltransferase 1 (soluble)
• Calculated Molecular Weight: 483 aa, 53 kDa
• Observed Molecular Weight: 45-53 kDa
• GenBank Accession Number: BC038598
• Gene Symbol: SHMT1
• Gene ID (NCBI): 6470
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SHMT1 is the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. This antibody can detect both 49 kDa and 53 kDa isoforms in western blotting.