Shh Recombinant antibody

Shh Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240057G4

Cat no : 83280-2-RR

Synonyms

9530036O11Rik, Dsh, Hhg1, Hx, Hxl3, M100081, Shh, sonic hedgehog

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Validation Data Gallery

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  WB analysis using 83280-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83280-2-RR (SHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, C2C12 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83280-2-RR is Shh Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: sonic hedgehog
• Calculated Molecular Weight: 48KD
• Observed Molecular Weight: 19-50 kDa
• GenBank Accession Number: NM_009170.3
• Gene Symbol: Shh
• Gene ID (NCBI): 20423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.