SHC1 Polyclonal antibody, PBS Only

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  WB analysis using 12496-1-AP (same clone as 12496-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  WB result of SHC antibody (12496-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-SHC transfected HeLa cells. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12496-1-AP (SHC antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 12496-1-AP (same clone as 12496-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12496-1-AP (SHC antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12496-1-AP (same clone as 12496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12496-1-AP (same clone as 12496-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SHC antibody (12496-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 12496-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SHC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12496-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SHC, SH2 domain protein C1, SHC-transforming protein A, SHC-transforming protein 3, SHC-transforming protein 1

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Product Information

12496-1-PBS targets SHC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC1 fusion protein Ag3165
• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66 kDa, 52 kDa, 46 kDa
• GenBank Accession Number: BC014158
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• RRID: AB_2187889
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P29353
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.