SH3GLB2 Monoclonal antibody, PBS Only

SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4B1A10

Cat no : 68325-1-PBS

Synonyms

Endophilin B2, KIAA1848, PP6569, PP9455, SH3GLB2

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Validation Data Gallery

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  WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68325-1-Ig (same clone as 68325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

  IP result of anti-SH3GLB2 (IP:68325-1-Ig, 4ug; Detection:68325-1-Ig 1:800) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68325-1-Ig (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68325-1-Ig (same clone as 68325-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SH3GLB2 antibody (68325-1-Ig, Clone: 4B1A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68325-1-PBS in a different storage buffer formulation.

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Product Information

68325-1-PBS targets SH3GLB2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: Human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SH3GLB2 fusion protein Ag8932
• Full Name: SH3-domain GRB2-like endophilin B2
• Calculated Molecular Weight: 395 aa, 44 kDa
• Observed Molecular Weight: 47-50 kDa
• GenBank Accession Number: BC014635
• Gene Symbol: SH3GLB2
• Gene ID (NCBI): 56904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).