SH3GLB2 Monoclonal antibody
SH3GLB2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
4B1A10
Cat no : 68325-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | LNCaP cells, rat brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rabbit brain tissue, mouse brain tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
68325-1-Ig targets SH3GLB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.
Tested Reactivity | Human, mouse, rat, pig, rabbit |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | SH3GLB2 fusion protein Ag8932 |
Full Name | SH3-domain GRB2-like endophilin B2 |
Calculated Molecular Weight | 395 aa, 44 kDa |
Observed Molecular Weight | 47-50 kDa |
GenBank Accession Number | BC014635 |
Gene Symbol | SH3GLB2 |
Gene ID (NCBI) | 56904 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).
Protocols
Product Specific Protocols | |
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WB protocol for SH3GLB2 antibody 68325-1-Ig | Download protocol |
IHC protocol for SH3GLB2 antibody 68325-1-Ig | Download protocol |
IF protocol for SH3GLB2 antibody 68325-1-Ig | Download protocol |
IP protocol for SH3GLB2 antibody 68325-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |