SGTA Polyclonal antibody, PBS Only

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  WB analysis of K-562 using 11019-2-AP (same clone as 11019-2-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  WB analysis of human skeletal muscle using 11019-2-AP (same clone as 11019-2-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 11019-2-AP (same clone as 11019-2-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 11019-2-AP (same clone as 11019-2-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

  IP result of anti-SGTA (IP:11019-2-AP, 4ug; Detection:11019-2-AP 1:1000) with HeLa cells lysate 2480ug. This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 11019-2-AP (same clone as 11019-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11019-2-AP (SGTA antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11019-2-AP (same clone as 11019-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11019-2-AP (same clone as 11019-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11019-2-AP (same clone as 11019-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 11019-2-AP (same clone as 11019-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11019-2-AP (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 11019-2-AP (same clone as 11019-2-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SGTA antibody (11019-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11019-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SGTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11019-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

11019-2-PBS targets SGTA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGTA fusion protein Ag1475
• Full Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC008885
• Gene Symbol: SGTA
• Gene ID (NCBI): 6449
• RRID: AB_2188830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43765
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.