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  • KD/KO Validated

SGTA Polyclonal antibody

SGTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11019-2-AP

Synonyms

SGT, hSGT, aSGT, Alpha-SGT, alphaSGT



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11019-2-AP targets SGTA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SGTA fusion protein Ag1475
Full Name small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34-37 kDa
GenBank Accession NumberBC008885
Gene Symbol SGTA
Gene ID (NCBI) 6449
RRIDAB_2188830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

Protocols

Product Specific Protocols
WB protocol for SGTA antibody 11019-2-APDownload protocol
IHC protocol for SGTA antibody 11019-2-APDownload protocol
IF protocol for SGTA antibody 11019-2-APDownload protocol
IP protocol for SGTA antibody 11019-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

J Cell Biol

Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.

Authors - Yu-Jie Chen
humanWB

PLoS Pathog

A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.

Authors - Christopher Paul Walczak
  • KD Validated
WB

PLoS Pathog

A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.

Authors - Madhu Sudhan Ravindran
humanIHC

Int J Cancer

Knockdown of the co-chaperone SGTA results in the suppression of androgen and PI3K/AKT signaling and inhibition of prostate cancer cell proliferation.

Authors - Trotta Andrew P AP
mouseWB

Int J Mol Sci

Isoquercitrin Attenuates Steatohepatitis by Inhibition of the Activated NLRP3 Inflammasome through HSP90

Authors - Ji Ma
mouseIHC

Sci Rep

Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice.

Authors - Lisa K Philp
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

WEI (Verified Customer) (03-08-2022)

Specific band at predicted size.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart
FH

WEI (Verified Customer) (11-12-2020)

A strong band at the predicted ~35kDa size.

  • Applications: Western Blot
  • Cell Tissue Type: Mouse hearts
FH

Tom (Verified Customer) (10-09-2020)

I saw a band at ~36kDa using this antibody. There are some non-specific bands present but the specific SGTA band was the strongest band.

  • Applications: Western Blot