SGTA Polyclonal antibody

• Featured Product
• KD/KO Validated

SGTA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (3)

Cat no : 11019-2-AP

Synonyms

SGT, hSGT, aSGT, Alpha-SGT, alphaSGT

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Validation Data Gallery

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  WB analysis of K-562 using 11019-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11019-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11019-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 11019-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11019-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11019-2-AP (SGTA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11019-2-AP

  IP result of anti-SGTA (IP:11019-2-AP, 4ug; Detection:11019-2-AP 1:1000) with HeLa cells lysate 2480ug.

• 

  IHC staining of human lung cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11019-2-AP (SGTA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11019-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11019-2-AP (SGTA Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11019-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 11019-2-AP (SGTA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 11019-2-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SGTA antibody (11019-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, human skeletal muscle tissue, mouse liver tissue, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lung cancer tissue, human liver cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 8 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

11019-2-AP targets SGTA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGTA fusion protein Ag1475
• Full Name: small glutamine-rich tetratricopeptide repeat (TPR)-containing, alpha
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34-37 kDa
• GenBank Accession Number: BC008885
• Gene Symbol: SGTA
• Gene ID (NCBI): 6449
• RRID: AB_2188830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) is a steroid receptor molecular co-chaperone critical in a range of biological processes, including viral infection, cell division, mitosis, and cell cycle checkpoint activation. Overexpression of SGTA has been found in various cancers, including prostate/ovary/testis/liver/lung cancer.

Publications

Species	Application	Title
	WB	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak ; KD Validated
	WB	PLoS Pathog; A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.; Authors - Madhu Sudhan Ravindran
human	IHC	Int J Cancer; Knockdown of the co-chaperone SGTA results in the suppression of androgen and PI3K/AKT signaling and inhibition of prostate cancer cell proliferation.; Authors - Trotta Andrew P AP
mouse	WB	Int J Mol Sci; Isoquercitrin Attenuates Steatohepatitis by Inhibition of the Activated NLRP3 Inflammasome through HSP90; Authors - Ji Ma
mouse	IHC	Sci Rep; Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice.; Authors - Lisa K Philp ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (03-08-2022)

Specific band at predicted size.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

FH

WEI (Verified Customer) (11-12-2020)

A strong band at the predicted ~35kDa size.

• Applications: Western Blot
• Cell Tissue Type: Mouse hearts

FH

Tom (Verified Customer) (10-09-2020)

I saw a band at ~36kDa using this antibody. There are some non-specific bands present but the specific SGTA band was the strongest band.

• Applications: Western Blot