SFRP2 Monoclonal antibody, PBS Only

SFRP2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3A1

Cat no : 66328-1-PBS

Synonyms

FRP 2, FRP2, SARP 1, SARP1, SDF 5, sFRP 2, SFRP2, UNQ361/PRO697

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Validation Data Gallery

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  WB analysis using 66328-1-Ig (same clone as 66328-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 66328-1-Ig (same clone as 66328-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 66328-1-Ig (same clone as 66328-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66328-1-Ig (same clone as 66328-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IHC staining of human malignant melanoma using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66328-1-Ig (SFRP2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66328-1-Ig (same clone as 66328-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SFRP2 antibody (66328-1-Ig, Clone: 1F3A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66328-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66328-1-PBS targets SFRP2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, rabbit, mouse samples.

• Tested Reactivity: human, rat, pig, rabbit, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SFRP2 fusion protein Ag18840
• Full Name: secreted frizzled-related protein 2
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC008666
• Gene Symbol: SFRP2
• Gene ID (NCBI): 6423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.