SFPQ Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SFPQ Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G4A5

Cat no : 67129-1-PBS

Synonyms

POMP100, POMP 100, Polypyrimidine tract-binding protein-associated-splicing factor, DNA-binding p52/p100 complex, 100 kDa subunit, 1G4A5

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Validation Data Gallery

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  WB analysis using 67129-1-Ig (same clone as 67129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67129-1-Ig (same clone as 67129-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

  WB result of SFPQ antibody (67129-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SFPQ transfected HeLa cells. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67129-1-Ig (same clone as 67129-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 67129-1-Ig (same clone as 67129-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67129-1-Ig (same clone as 67129-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67129-1-Ig (same clone as 67129-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67129-1-Ig (same clone as 67129-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67129-1-Ig (same clone as 67129-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67129-1-Ig (SFPQ antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 67129-1-Ig (SFPQ antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67129-1-Ig (same clone as 67129-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SFPQ antibody (67129-1-Ig, Clone: 1G4A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67129-1-Ig (same clone as 67129-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SFPQ (67129-1-Ig, Clone:1G4A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67129-1-PBS in a different storage buffer formulation.

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Product Information

67129-1-PBS targets SFPQ in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SFPQ fusion protein Ag7181
• Full Name: splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC051192
• Gene Symbol: SFPQ
• Gene ID (NCBI): 6421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SFPQ, also named PSF, encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples. In summary, our study implicates SFPQ IR and nuclear loss as general molecular hallmarks of familial and sporadic ALS.