CoraLite® Plus 488-conjugated SERPINB9 Monoclonal antibody

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  IF Staining of HeLa using CL488-67950

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 SERPINB9 antibody (CL488-67950, Clone: 2H9D2 ) at dilution of 1:200.

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  IF Staining of C6 using CL488-67950

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CoraLite® Plus 488 SERPINB9 antibody (CL488-67950, Clone: 2H9D2 ) at dilution of 1:200.

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  FC experiment of K-562 using CL488-67950

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SERPINB9 (CL488-67950, Clone:2H9D2) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SERPINB9 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. CL488-67950

Clone No.2H9D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

PI9, PI 9, CAP-3, CAP3, CAP 3

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Tested Applications

• Positive IF/ICC detected in: HeLa cells, C6 cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67950 targets SERPINB9 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SERPINB9 fusion protein Ag6719
• Full Name: serpin peptidase inhibitor, clade B (ovalbumin), member 9
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC002538
• Gene Symbol: SERPINB9
• Gene ID (NCBI): 5272
• RRID: AB_2923839
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P50453
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINB9, also named as PI-9, belongs to the serpin family. SERPINB9 is an intracellular inhibitor of granzyme B (grB) that protects cytotoxic lymphocytes from grB-mediated death. It has a nucleo-cytoplasmic distribution and is produced in CD8+ T cells and natural killer cells (PMID: 28024184). Also, SERPINB9 is expressed by vascular SMCs and endothelial cells to avoid GZB-induced apoptosis (PMID: 28743062). The molecular weight of SERPINB9 is 42 kDa.