Protein C inhibitor Monoclonal antibody

Protein C inhibitor Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H6G2

Cat no : 66030-1-Ig

Synonyms

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

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Validation Data Gallery

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  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Human testis, tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human ovary tumor tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66030-1-Ig is Protein C inhibitor Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Protein C inhibitor fusion protein Ag18094
• Full Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC008915
• Gene Symbol: SERPINA5
• Gene ID (NCBI): 5104
• RRID: AB_11045661
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.