CoraLite®594-conjugated Septin 2 Monoclonal antibody

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  IF Staining of K-562 using CL594-60075

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-60075

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL594-60075

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 Septin 2 antibody (CL594-60075, Clone: 2F3B2 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

Septin 2 Monoclonal Antibody for IF/ICC

Cat No. CL594-60075

Clone No.2F3B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

NEDD-5, NEDD5, NEDD 5, KIAA0158, hNedd5

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells, HepG2 cells, K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60075 targets Septin 2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Septin 2 fusion protein Ag1946
• Full Name: septin 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC014455
• Gene Symbol: Septin 2
• Gene ID (NCBI): 4735
• RRID: AB_2883416
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15019
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Septin 2, also named as DIFF6, KIAA0158, NEDD5, belongs to the septin family. It is a filament-forming cytoskeletal GTPase. Septin 2 is required for normal organization of the actin cytoskeleton. It plays a role in ciliogenesis and collective cell movements. Septin 2 is coordinated expression with Septin 6 and Septin 7. (PMID:21737677)