SENP1 Recombinant antibody, PBS Only (Detector)

SENP1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240478F3

Cat no : 83594-1-PBS

Synonyms

SuPr2, SuPr 2, Sentrin specific protease 1, SENP 1, 240478F3

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Validation Data Gallery

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  IHC staining of human intrahepatic cholangiocarcinoma using 83594-1-RR (same clone as 83594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83594-1-RR (SENP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83594-1-RR (same clone as 83594-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SENP1 antibody (83594-1-RR, Clone: 240478F3 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83594-1-RR (same clone as 83594-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SENP1 Recombinant antibody (83594-1-RR, Clone:240478F3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83594-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00607-1

  Cytometric bead array standard curve of MP00607-1, SENP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83594-2-PBS. Detection antibody: 83594-1-PBS. Standard: Ag18028. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83594-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83594-1-RR against Human SENP1 were performed. The affinity constant is 1.91 nM.

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Product Information

83594-1-PBS targets SENP1 as part of a matched antibody pair:

MP00607-1: 83594-2-PBS capture and 83594-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SENP1 fusion protein Ag18028
• Full Name: SUMO1/sentrin specific peptidase 1
• Calculated Molecular Weight: 644 aa, 73 kDa
• GenBank Accession Number: BC045639
• Gene Symbol: SENP1
• Gene ID (NCBI): 29843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SENP1, Sentrin-specific protease 1, is a cysteine protease that specifically targets members of the small ubiquitin-like modifier (SUMO) protein family. SENP1 catalyzes two functions in the SUMO pathway (PMID: 29506078).The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins (PMID: 16253240). SENP1 is located in the nucleus and cytosol.