SEMA7A Monoclonal antibody

SEMA7A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3A5G1

Cat no : 67397-1-Ig

Synonyms

CD108, CDw108, H SEMA K1, H Sema L, JMH, JMH blood group antigen, Sema K1, Sema L, SEMA7A, SEMAK1, SEMAL, Semaphorin 7A, Semaphorin K1, Semaphorin L

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Validation Data Gallery

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  WB analysis of human red blood cells using 67397-1-Ig

  human red blood cells were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67397-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67397-1-Ig (SEMA7A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human red blood cells, human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

67397-1-Ig targets SEMA7A in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEMA7A fusion protein Ag28804
• Full Name: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• Calculated Molecular Weight: 666 aa, 75 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC101643
• Gene Symbol: SEMA7A
• Gene ID (NCBI): 8482
• RRID: AB_2882640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEMA7A, also named CD108 and SEMAL, belongs to the semaphorin family. SEMA7A is the only membrane‐associated glycosylphosphatidylinositol (GPI)‐linked semaphorin and plays a role in the regulation of neuronal axon outgrowth. In addition to its role in guiding axon pathfinding during neuronal development, Sema7A has diverse functions in morphogenesis and immune cell control and regulates T cell responses via the α1β1 integrin receptor (PMID: 31179640). SEMA7A has 2 isoforms with molecular weights of 73 and 75 kDa, respectively.

Publications

Species	Application	Title
mouse	WB	Cell Commun Signal; Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation; Authors - Xuan Li