FcZero-rAb™ PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody

SELPLG/CD162 FcZero-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241297F10

Cat no : PE-FcA98084

Synonyms

SELPLG, PSGL1, PSGL 1, P-selectin glycoprotein ligand 1, CLA

---

Validation Data Gallery

• 

  FC experiment of human peripheral blood leukocyte using PE-FcA98084

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9). Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocyte using PE-FcA98084

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human SELPLG/CD162 Rabbit Recombinant Antibody (PE-FcA98084, Clone: 241297F10) (red) or 5 ul PE Rabbit IgG Isotype Control Recombinant Antibody (PE-FcA98136, Clone: 240953C9) (blue). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-FcA98084 targets SELPLG/CD162 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: selectin P ligand
• Calculated Molecular Weight: 412 aa, 43 kDa
• GenBank Accession Number: BC029782
• Gene Symbol: SELPLG
• Gene ID (NCBI): 6404
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

SELPLG (selectin P ligand), also known as CD162 or PSGL-1, is a 120 kDa type I transmembrane mucin-like glycoprotein. It is an adhesion molecule expressed on the surface of neutrophils, monocytes, and most lymphocytes as a homodimer of approximately 230-250 kDa (PMID: 7545173; 1378449; 9353350). SELPLG plays a critical role in the early stages of inflammation due to its ability to bind E-selectin (CD62E), L-selectin (CD62L), and P-selectin (CD62P) (PMID: 35597982).