SEC61B-Specific Polyclonal antibody, PBS Only

SEC61B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14846-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 14846-1-AP (same clone as 14846-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 14846-1-AP (same clone as 14846-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14846-1-AP (same clone as 14846-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

---

Product Information

14846-1-PBS targets SEC61B-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Sec61 beta subunit
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: NM_006808
• Gene Symbol: SEC61B
• Gene ID (NCBI): 10952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).