Filter: AllWBIHCIF-PIF/ICC

WB analysis using 14846-1-AP (same clone as 14846-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 14846-1-AP (same clone as 14846-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 14846-1-AP (same clone as 14846-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 14846-1-AP (SEC61B-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of human cervical cancer using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14846-1-AP (same clone as 14846-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14846-1-AP (SEC61B-Specific Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IF Staining of mouse pancreas using 14846-1-AP (same clone as 14846-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 14846-1-AP (same clone as 14846-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SEC61B-Specific antibody (14846-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14846-1-PBS in a different storage buffer formulation.

SEC61B-Specific Polyclonal antibody, PBS Only

SEC61B-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 14846-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14846-1-PBS targets SEC61B-Specific in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	Sec61 beta subunit
Calculated Molecular Weight	10 kDa
Observed Molecular Weight	10-15 kDa
GenBank Accession Number	NM_006808
Gene Symbol	SEC61B
Gene ID (NCBI)	10952
RRID	AB_2270168
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P60468
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).