SEC61B Polyclonal antibody, PBS Only

SEC61B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51020-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 51020-2-AP (same clone as 51020-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 51020-2-AP (SEC61B antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 51020-2-AP (same clone as 51020-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 51020-2-AP (SEC61B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using SEC61B antibody (51020-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 51020-2-AP (same clone as 51020-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 51020-2-AP (SEC61B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51020-2-PBS in a different storage buffer formulation.

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Product Information

51020-2-PBS targets SEC61B in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Sec61 beta subunit
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10-15 kDa
• GenBank Accession Number: BC001734
• Gene Symbol: SEC61B
• Gene ID (NCBI): 10952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The heteromeric SEC61 complex is composed of alpha, beta and gamma subunits. Oligomers of the SEC61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane (PMID: 10212142). SEC61B is the beta subunit of the SEC61 complex. It has been reported that SEC61B facilitates cotranslational protein transport and interacts with the signal peptidase during translocation (PMID: 9585408).