SEC5/EXOC2 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 66011-1-Ig (same clone as 66011-1-PBS)

  WB result of SEC5/EXOC2 antibody (66011-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SEC5/EXOC2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  WB analysis of rat cerebellum using 66011-1-Ig (same clone as 66011-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66011-1-Ig (same clone as 66011-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 66011-1-Ig (same clone as 66011-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66011-1-Ig (same clone as 66011-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66011-1-Ig (same clone as 66011-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 66011-1-Ig (same clone as 66011-1-PBS)

  IP result of anti-SEC5/EXOC2 (IP:66011-1-Ig, 5ug; Detection:66011-1-Ig 1:500) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IF Staining of MDA-MB-231 using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunostaining of exocyst protein Sec5 (66011-1-Ig, red) in MDA-MB-231 cells. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66011-1-Ig (same clone as 66011-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC5/EXOC2 antibody (66011-1-Ig, Clone: 1C11G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66011-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

SEC5/EXOC2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 66011-1-PBS

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p

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Product Information

66011-1-PBS targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC5/EXOC2 fusion protein Ag17866
• Full Name: exocyst complex component 2
• Calculated Molecular Weight: 924 aa, 104 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC016918
• Gene Symbol: SEC5
• Gene ID (NCBI): 55770
• RRID: AB_11042317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96KP1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.