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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SEC31A Polyclonal antibody

SEC31A Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17913

Synonyms

SEC31L1, SEC31 related protein A, SEC31 like protein 1, KIAA0905, ABP130

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SEC31A Polyclonal an (CL488-17913)

    IF Staining of HeLa using CL488-17913

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SEC31A antibody (CL488-17913) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated SEC31A Polyclonal an (CL488-17913)

    IF Staining of HeLa using CL488-17913

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SEC31A antibody (CL488-17913) at dilution of 1:200, CL594-Phalloidin (red).

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17913 targets SEC31A in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC31A fusion protein Ag12314
Full Name SEC31 homolog A (S. cerevisiae)
Calculated Molecular Weight 1220 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC084583
Gene Symbol SEC31A
Gene ID (NCBI) 22872
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec31 subunit of the coat complex has been shown to be essential for vesicle formation and ER-Golgi transport (PMID: 10788476). Two mammalian homologues of yeast sec31p, Sec31A and Sec31B, share about 40% amino acid identity. Sec31A transcripts are ubiquitously and abundantly expressed.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SEC31A antibody CL488-17913Download protocol
Standard Protocols
Click here to view our Standard Protocols