CoraLite® Plus 488-conjugated SEC24A Recombinant antibody

SEC24A Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230105B3

Cat no : CL488-82908

Synonyms

SEC24-related protein A, Protein transport protein Sec24A, 230105B3

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Validation Data Gallery

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  IF Staining of HeLa using CL488-82908

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SEC24A antibody (CL488-82908, Clone: 230105B3 ) at dilution of 1:200.

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  FC experiment of Hela using CL488-82908

  1x10^6 Hela cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sec24a Recombinant Antibody (CL488-82908, Clone:230105B3)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: Hela cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82908 targets SEC24A in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SEC24A fusion protein Ag8723
• Full Name: SEC24 family, member A (S. cerevisiae)
• Calculated Molecular Weight: 613 aa, 66 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC019341
• Gene Symbol: SEC24A
• Gene ID (NCBI): 10802
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells, there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445). This antibody is specific to SEC24A.