SDS Polyclonal antibody, PBS Only

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  WB analysis using 16594-1-AP (same clone as 16594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16594-1-AP (same clone as 16594-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16594-1-AP (same clone as 16594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16594-1-AP (same clone as 16594-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16594-1-PBS in a different storage buffer formulation.

SDS Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16594-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16594-1-PBS targets SDS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDS fusion protein Ag9704
• Full Name: serine dehydratase
• Calculated Molecular Weight: 218 aa, 23 kDa
• Observed Molecular Weight: 35 kDa; 70 kDa
• GenBank Accession Number: BC020750
• Gene Symbol: SDS
• Gene ID (NCBI): 10993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20132
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)