SDS Polyclonal antibody

SDS Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16594-1-AP

Synonyms

L serine deaminase, L threonine dehydratase, SDH, SDS, serine dehydratase, TDH

---

Validation Data Gallery

• 

  WB analysis using 16594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16594-1-AP (SDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16594-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16594-1-AP (SDS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16594-1-AP targets SDS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SDS fusion protein Ag9704
• Full Name: serine dehydratase
• Calculated Molecular Weight: 218 aa, 23 kDa
• Observed Molecular Weight: 35 kDa; 70 kDa
• GenBank Accession Number: BC020750
• Gene Symbol: SDS
• Gene ID (NCBI): 10993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L-Serine dehydratase (SDS ; SDH) catalyzes the pyridoxal phosphate (PLP) dependent deamination of l-serine to yield pyruvate. In mammals, SDH is found predominantly in the liver. Extensive studies have been carried out on SDH from rat and the enzyme has been found to play an important role in gluconeogenesis; its activity is induced by the consumption of high-protein diets, starvation and other treatments. The enzyme exists as a dimer of identical subunits, with each subunit exhibiting a bilobal architecture. (PMID: 25380533, PMID: 21878319, PMID: 14646100)