CoraLite®594-conjugated SDHB Monoclonal antibody

SDHB Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A2A5

Cat no : CL594-67600

Synonyms

FLJ92337, IP, PGL4, SDH, SDH1, SDHB, SDHIP

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-67600

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 SDHB antibody (CL594-67600, Clone: 1A2A5 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67600 targets SDHB in IF-P applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDHB fusion protein Ag29868
• Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC007840
• Gene Symbol: SDHB
• Gene ID (NCBI): 6390
• RRID: AB_2920162
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).