SDHB Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SDHB Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A2A5

Cat no : 67600-1-PBS

Synonyms

FLJ92337, IP, PGL4, SDH, SDH1, SDHB, SDHIP

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Validation Data Gallery

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  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 67600-1-Ig (same clone as 67600-1-PBS)

  WB result of SDHB antibody (67600-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SDHB transfected HepG2 cells. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67600-1-Ig (same clone as 67600-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67600-1-Ig (SDHB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67600-1-Ig (SDHB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 67600-1-Ig (same clone as 67600-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using SDHB antibody (67600-1-Ig, Clone: 1A2A5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67600-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67600-1-PBS targets SDHB in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDHB fusion protein Ag29868
• Full Name: succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC007840
• Gene Symbol: SDHB
• Gene ID (NCBI): 6390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).