SDCCAG1 Monoclonal antibody

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  WB analysis using 68708-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68708-1-Ig (SDCCAG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SDCCAG1 Monoclonal Antibody for WB, ELISA

Cat No. 68708-1-Ig

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

Ribosome quality control complex subunit NEMF, NY CO 1, Nuclear export mediator factor, NEMF, Homo sapiens (Human)

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Tested Applications

• Positive WB detected in: U2OS cells, A549 cells, LNCaP cells, HeLa cells, MCF-7 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68708-1-Ig targets SDCCAG1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SDCCAG1 fusion protein Ag2467
• Full Name: serologically defined colon cancer antigen 1
• Calculated Molecular Weight: 1076 aa, 123 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC020794
• Gene Symbol: SDCCAG1
• Gene ID (NCBI): 9147
• RRID: AB_3670401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O60524
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).