SCNN1G Polyclonal antibody

SCNN1G Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30037-1-AP

Synonyms

Gamma-ENaC, Gamma NaCH, Gamma ENaC, ENaCgamma, ENaCg

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Validation Data Gallery

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  WB analysis using 30037-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30037-1-AP (SCNN1G antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A431 cells, COLO 320 cells, PC-3 cells, pig kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30037-1-AP targets SCNN1G in WB, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCNN1G fusion protein Ag32176
• Full Name: sodium channel, nonvoltage-gated 1, gamma
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC059391
• Gene Symbol: SCNN1G
• Gene ID (NCBI): 6340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCNN1G (sodium channel, non-voltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of the kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and may undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).