CoraLite® Plus 488-conjugated SCN5A Monoclonal antibody

SCN5A Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Rabbit

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C2B3

Cat no : CL488-68273

Synonyms

Nav1.5, HH1, CMPD2, CMD1E, CDCD2

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Validation Data Gallery

• 

  FC experiment of HeLa using CL488-68273

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SCN5A (CL488-68273, Clone:1C2B3) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68273 targets SCN5A in FC (Intra) applications and shows reactivity with Human, Rat, Rabbit samples.

• Tested Reactivity: Human, Rat, Rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SCN5A fusion protein Ag19275
• Full Name: sodium channel, voltage-gated, type V, alpha subunit
• Calculated Molecular Weight: 2016 aa, 227 kDa
• Observed Molecular Weight: 227 kDa
• GenBank Accession Number: BC140813
• Gene Symbol: SCN5A
• Gene ID (NCBI): 6331
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).