CoraLite® Plus 750-conjugated SCN3B Recombinant antibody

SCN3B Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230316B2

Cat no : CL750-82959-7

Synonyms

HSA243396, KIAA1158, SCN3B, SCNB3, Sodium channel subunit beta 3

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Validation Data Gallery

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  WB analysis using CL750-82959-7

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-82959-7 (SCN3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL750-82959-7 is SCN3B Fusion Protein expressed in E. coli.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCN3B fusion protein Ag23201
• Full Name: sodium channel, voltage-gated, type III, beta
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC126265
• Gene Symbol: SCN3B
• Gene ID (NCBI): 55800
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The sodium channel is a multi-subunit protein complex composed of a single large α-subunit along with smaller additional β-subunits. There are at least three different β-subunit genes, SCN1b, SCN2b, and SCN3b, all of which are expressed widely in excitable tissues. The bands on Western blots for SCN3b (45-50 kDa) were significantly larger than the predicted molecular weight, which is consistent with the protein being glycosylated. The two bands on the Western blot could be due to different levels of glycosylation or alternative spliced isoforms. (PMID: 11744748)