SCD1 Recombinant antibody, PBS Only

SCD1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241487E12

Cat no : 81468-5-PBS

Synonyms

SCD, Delta-9 desaturase, Delta(9)-desaturase, Delta(9) desaturase, Acyl-CoA desaturase

---

Validation Data Gallery

• 

  WB analysis using 81468-5-RR (same clone as 81468-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81468-5-RR (SCD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 81468-5-RR (same clone as 81468-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (81468-5-RR, Clone: 241487E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 81468-5-RR (same clone as 81468-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 81468-5-RR (same clone as 81468-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug SCD Recombinant antibody (81468-5-RR, Clone:241487E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81468-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 81468-5-RR

  Biolayer interferometry (BLl) kinetic assays of 81468-5-RR against Human SCD were performed. The affinity constant is 1.83 nM.

---

Product Information

The immunogen of 81468-5-PBS is SCD1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCD1 fusion protein Ag29156
• Full Name: stearoyl-CoA desaturase (delta-9-desaturase)
• Calculated Molecular Weight: 355 aa, 41 kDa
• Observed Molecular Weight: 28-42 kDa
• GenBank Accession Number: BC005807
• Gene Symbol: SCD
• Gene ID (NCBI): 6319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).