SAT1 Recombinant antibody, PBS Only (Detector)

SAT1 Recombinant Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240157G2

Cat no : 83319-1-PBS

Synonyms

DC21, Diamine acetyltransferase 1, KFSD, Putrescine acetyltransferase, SAT, SAT1, SSAT, SSAT 1

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Validation Data Gallery

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  WB analysis using 83319-1-RR (same clone as 83319-1-PBS)

  Untreated and DENSPM treated HEK-293 and HeLa cells were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 83319-1-RR (same clone as 83319-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83319-1-RR (SAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 83319-1-RR (same clone as 83319-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SAT1 antibody (83319-1-RR, Clone: 240157G2 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83319-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00344-2

  Cytometric bead array standard curve of MP00344-2, SAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83319-3-PBS. Detection antibody: 83319-1-PBS. Standard: Ag1154. Range: 0.625-80 ng/mL.

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  Cytometric bead array standard curve of MP00344-3

  Cytometric bead array standard curve of MP00344-3, SAT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83319-2-PBS. Detection antibody: 83319-1-PBS. Standard:null. Range: null.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83319-1-PBS targets SAT1 as part of a matched antibody pair:

MP00344-2: 83319-3-PBS capture and 83319-1-PBS detection (validated in Cytometric bead array)

MP00344-3: 83319-2-PBS capture and 83319-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SAT1 fusion protein Ag1154
• Full Name: spermidine/spermine N1-acetyltransferase 1
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC008424
• Gene Symbol: SAT1
• Gene ID (NCBI): 6303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.