Virus SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody, PBS Only (Capture)

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Virus

Applications

WB, ELISA, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B3C3

Cat no : 67666-1-PBS

Synonyms

2019-nCOV nucleocapsid phosphoprotein, COVID-19 N protein, N protein, nucleocapsid phosphoprotein, SARS-COV-2 N protein, SARS-COV-2 nucleocapsid phosphoprotein

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Validation Data Gallery

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  WB analysis using 67666-1-Ig (same clone as 67666-1-PBS)

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50061-1

  Sandwich ELISA standard curve of MP50061-1, Virus 2019-nCOV nucleocapsid phosphoprotein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67666-1-PBS. Detection antibody: HRP-conjugated 67666-2-PBS. Standard: Ag30676. Range: 0.5-20 ng/mL.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

• 

  ELISA experiment of Recombinant protein using 67666-1-Ig (same clone as 67666-1-PBS)

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 67666-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67666-1-PBS targets SARS-CoV-2 Nucleocapsid Phosphoprotein as part of a matched antibody pair:

MP50061-1: 67666-1-PBS capture and 67666-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: Virus
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Full Name: COVID-19 N Protein
• GenBank Accession Number: NC_045512
• Gene Symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.