SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Virus and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1B3C3

Cat no : 67666-1-Ig

Synonyms

2019-nCOV nucleocapsid phosphoprotein, COVID-19 N protein, N protein, nucleocapsid phosphoprotein, SARS-COV-2 N protein, SARS-COV-2 nucleocapsid phosphoprotein

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Validation Data Gallery

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  WB analysis using 67666-1-Ig

  E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 67666-1-Ig and 67666-2-Ig at various work concentration.

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  ELISA experiment of Recombinant protein using 67666-1-Ig

  Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 67666-1-Ig and 67666-2-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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  ELISA experiment of Recombinant protein using 67666-1-Ig

  Sandwich ELISA was carried out by coating 67666-1-Ig at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated clone 67666-2-Ig was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

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Tested Applications

• Positive WB detected in: Ag30676
• Positive ELISA detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67666-1-Ig targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with Virus samples.

• Tested Reactivity: Virus
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
• Full Name: COVID-19 N Protein
• GenBank Accession Number: NC_045512
• Gene Symbol: COVID-19 N Protein
• Gene ID (NCBI): 43740575
• RRID: AB_2882862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387).67666-1-Ig can be used as capture antibody. 67666-2-Ig can be used as detection antibody.

Publications

Species	Application	Title
mouse		Biosens Bioelectron; Electronic field effect detection of SARS-CoV-2 N-protein before the onset of symptoms.; Authors - I Novodchuk
		Viruses; Collection of Monoclonal Antibodies Targeting SARS-CoV-2 Proteins.; Authors - Marina Pribanić Matešić
		J Virol; Palmitoylation of SARS-CoV-2 Envelope protein is central to virus particle formation; Authors - Zhaohuan Wang