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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated SAMHD1 Polyclonal antibody

SAMHD1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-12586

Synonyms

DCIP, HDDC1, Monocyte protein 5, MOP 5, MOP5, SAM domain and HD domain 1, SAMHD1, SBBI88

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A549 cells using CoraLite®594-conjugated SAMHD1 Polyclonal antibody (CL594-12586)

    IF Staining of A549 using CL594-12586

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 SAMHD1 antibody (CL594-12586) at dilution of 1:400.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SAMHD1 Polyclonal antibody (CL594-12586)

    IF Staining of HepG2 using CL594-12586

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 SAMHD1 antibody (CL594-12586) at dilution of 1:400.

Tested Applications

Positive IF/ICC detected inHepG2 cells, A549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-12586 targets SAMHD1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SAMHD1 fusion protein Ag3287
Full Name SAM domain and HD domain 1
Calculated Molecular Weight 626 aa, 72 kDa
Observed Molecular Weight 64-72 kDa
GenBank Accession NumberBC036450
Gene Symbol SAMHD1
Gene ID (NCBI) 25939
RRIDAB_2919785
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SAMHD1, highly expressed by monocytes and monocyte-derived dendritic cells and by monocyte-derived macrophages at a lower extent, is reported as an HIV-1 restriction factor that inhibits the early step of the HIV-1 life cycle. Vpx (virus-like particles containing viral protein X) could overcome this block by interacting with SAMHD1, inducing proteasome-dependent degradation of SAMHD1. Mutations in SAMHD1 cause Aicardi-Goutières syndrome, a genetic encephalopathy with a presumed immune pathogenesis. Three alternatively spliced transcripts encoding different isoforms have been described.

Protocols

Product Specific Protocols
IF protocol for CL594 SAMHD1 antibody CL594-12586Download protocol
FC protocol for CL594 SAMHD1 antibody CL594-12586Download protocol
Standard Protocols
Click here to view our Standard Protocols