SALL4 Recombinant antibody

SALL4 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230284C4

Cat no : 83039-4-RR

Synonyms

Zinc finger protein 797, Sal-like protein 4, Sal like protein 4, HSAL4, DRRS

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Validation Data Gallery

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  IF Staining of Caco-2 using 83039-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of Caco-2 using 83039-4-RR

  Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SALL4 antibody (83039-4-RR, Clone: 230284C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 83039-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83039-4-RR against Human SALL4 were performed. The affinity constant is 0.18 nM.

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Tested Applications

• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83039-4-RR targets SALL4 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SALL4 fusion protein Ag16076
• Full Name: sal-like 4 (Drosophila)
• Calculated Molecular Weight: 1053 aa, 112 kDa
• GenBank Accession Number: BC111714
• Gene Symbol: SALL4
• Gene ID (NCBI): 57167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SALL4, also named Sal-like protein 4 or Zinc finger protein 797, Contains 7 C2H2-type zinc fingers and belongs to the sal C2H2-type zinc-finger protein family. SALL4 is constitutively expressed in acute myeloid leukemia. The constitutive expression of SALL4 in mice is sufficient to induce MDS-like symptoms and transformation to AML that is transplantable. SALL4 is able to bind beta-catenin and activate the Wnt/beta-catenin signaling pathway. Sequence analysis of the larger cDNA fragment isolated revealed a single, large open-reading frame, designated as SALL4A, that started from a strong consensus initiation sequence and was expected to encode 1053 amino acids. The other splicing variant of SALL4, designated SALL4B, lacked the region corresponding to amino acids 385 to 820 of the full-length SALL4A. The putative protein encoded by SALL4B cDNA was expected to consist of 617 amino acids.