CoraLite® Plus 488-conjugated S1PR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

S1PR2 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-21180

Synonyms

AGR16, EDG 5, EDG5, Gpcr13, H218, LPB2, S1P receptor 2, S1P receptor Edg 5, S1P2, S1PR2

---

Validation Data Gallery

• 

  IF Staining of mouse brain using CL488-21180

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 S1PR2 antibody (CL488-21180) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-21180

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 S1PR2 antibody (CL488-21180) at dilution of 1:200.

---

Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-21180 is S1PR2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S1PR2 fusion protein Ag15470
• Full Name: sphingosine-1-phosphate receptor 2
• Calculated Molecular Weight: 353 aa, 39 kDa
• Observed Molecular Weight: 40-50 kDa
• GenBank Accession Number: BC069598
• Gene Symbol: S1PR2
• Gene ID (NCBI): 9294
• RRID: AB_2919178
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S1PR2 (sphingosine-1-phosphate receptor 2), also known as as S1P2 or EDG5, is one of five G-protein-coupled receptors for sphingosine-1-phosphate (S1P), a biologically active metabolic product of sphingolipid (PMID: 18787560). S1P and its receptors have important regulatory functions in normal physiology and disease processes, particularly involving the immune, central nervous, and cardiovascular systems (PMID: 21339489). S1PR2 plays a role in acute vascular inflammation (PMID: 23723450). S1PR2 signaling is implicated in STAT3 activation (PMID: 22606352).