S100P Monoclonal antibody, PBS Only

S100P Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G6C3

Cat no : 67485-1-PBS

Synonyms

MIG9, Protein S100 E, Protein S100 P, S100 calcium binding protein P, S100E, S100P

---

Validation Data Gallery

• 

  WB analysis of BXPC-3 using 67485-1-Ig (same clone as 67485-1-PBS)

  BXPC-3 cells were subjected to SDS PAGE followed by western blot with 67485-1-Ig (S100P antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human urothelial carcinoma using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67485-1-Ig (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 67485-1-Ig (same clone as 67485-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using S100P antibody (67485-1-Ig, Clone: 1G6C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67485-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67485-1-PBS targets S100P in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100P fusion protein Ag19115
• Full Name: S100 calcium binding protein P
• Calculated Molecular Weight: 95 aa, 11 kDa
• Observed Molecular Weight: 10 KDa
• GenBank Accession Number: BC006819
• Gene Symbol: S100P
• Gene ID (NCBI): 6286
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.