S100P Polyclonal antibody

S100P Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11803-1-AP

Synonyms

S100 calcium-binding protein P, S100 calcium binding protein P, Protein S100-P, Protein S100-E, Protein S100 P

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Validation Data Gallery

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  WB analysis of HeLa using 11803-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11803-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11803-1-AP (S100P antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human bowen disease using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11803-1-AP (S100P antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11803-1-AP (S100P Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human urothelial carcinoma using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 11803-1-AP (S100P antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 11803-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11803-1-AP (S100P antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 11803-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using S100P antibody (11803-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 11803-1-AP

  Immunofluorescent analysis of HepG2 cells, using S100P antibody 11803-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human colon cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 6 publications below

Product Information

11803-1-AP targets S100P in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: S100P fusion protein Ag2379
• Full Name: S100 calcium binding protein P
• Calculated Molecular Weight: 95 aa, 11 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC006819
• Gene Symbol: S100P
• Gene ID (NCBI): 6286
• RRID: AB_2184577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100P protein is a relatively small (95 amino acid) isoform of the S100 protein family that was first isolated from human placenta. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P protein is widely expressed in both normal and neoplastic tissues. It clearly shows ectopic expression in some cancers. Based on the high expression in certain tumors, S100P could represent a potential target for novel diagnostic and therapeutic applications.

Publications

Species	Application	Title
human,mouse	WB,IHC	Adv Sci (Weinh); RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation; Authors - Yu Sun
human	WB, IHC	Am J Cancer Res; High expression of S100P is associated with unfavorable prognosis and tumor progression in patients with epithelial ovarian cancer.; Authors - Xiangyu Wang
human	IHC	Proteomics Clin Appl; Identification of distinctive protein expression patterns in colorectal adenoma.; Authors - F Lam Francis F
human	IHC	Diagn Pathol; Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion.; Authors - Jung Min Jung MJ
human	WB	Exp Ther Med; Proof-of-concept study investigating the role of S100P-RAGE in nasopharyngeal carcinoma.; Authors - Chengyu Wang
human	WB	Technol Cancer Res Treat; LHPP Inhibits the Viability, Migration, and Proliferation of PDAC Cells and Significantly Affects the Expression of SDC1 and S100p; Authors - Zhaozhi Xia