S100A9 Recombinant antibody

S100A9 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240539F4

Cat no : 83578-6-RR

Synonyms

CFAG, Calprotectin L1H subunit, Calgranulin-B, Calgranulin B, CAGB

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Validation Data Gallery

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  IF Staining of RAW 264.7 using 83578-6-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using S100A9 antibody (83578-6-RR, Clone: 240539F4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of A431 using 83578-6-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  FC experiment of Jurkat using 83578-6-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug S100A9 Recombinant antibody (83578-6-RR, Clone:240539F4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 83578-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83578-6-RR against Human S100A9 were performed. The affinity constant is 1.19 nM.

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC (Intra) detected in: A431 cells, Jurkat cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83578-6-RR is S100A9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S100A9 fusion protein Ag25764
• Full Name: S100 calcium binding protein A9
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: BC047681
• Gene Symbol: S100A9
• Gene ID (NCBI): 6280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A9 is a calcium binding protein as a member of the S100 family of proteins. S100 proteins are low molecular weight (9 to 14 kDa) intracellular calcium-binding proteins that control key cellular pathways including regulation of the cytoskeleton, cell migration and adhesion, and host oxidative defense. S100A9 may exist as a homodimer, heterodimer (24 kDa) with an S100A8 partner (S100A8/A9), or as a heterotetramer (28 kDa) with an S100A8 partner(S100A8/A9). S100A8 and S100A9 are found intracellularly in granulocytes, monocytes, and early differentiation stages of macrophages.