S100A6 Monoclonal antibody, PBS Only

S100A6 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D11C8

Cat no : 66098-1-PBS

Synonyms

2A9, 5B10, CABP, CACY, Calcyclin, MLN 4, PRA, Protein S100 A6, S100A6

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Validation Data Gallery

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  WB analysis of HeLa using 66098-1-Ig (same clone as 66098-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66098-1-Ig (same clone as 66098-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66098-1-Ig (same clone as 66098-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using S100A6 antibody 66098-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66098-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66098-1-PBS targets S100A6 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A6 fusion protein Ag17606
• Full Name: S100 calcium binding protein A6
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC001431
• Gene Symbol: S100A6
• Gene ID (NCBI): 6277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis.