S100A6 Monoclonal antibody

S100A6 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3D11C8

Cat no : 66098-1-Ig

Synonyms

MLN 4, Growth factor-inducible protein 2A9, Calcyclin, CACY, CABP

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Validation Data Gallery

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  WB analysis of HeLa using 66098-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66098-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66098-1-Ig (S100A6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66098-1-Ig (S100A6 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human colon cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IHC staining of human liver cancer using 66098-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66098-1-Ig (S100A6 Antibody) at dilution of 1:50.

• 

  IF Staining of HepG2 using 66098-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using S100A6 antibody (66098-1-Ig, Clone: 3D11C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

66098-1-Ig targets S100A6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: S100A6 fusion protein Ag17606
• Full Name: S100 calcium binding protein A6
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC001431
• Gene Symbol: S100A6
• Gene ID (NCBI): 6277
• RRID: AB_2881497
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S100A6 is also named as calcyclin, prolactin receptor-associated protein (PRA), growth factor-inducible protein 2A9 ir MLN4. It belongs to S100 family of low molecular weight, acidic, calcium-binding proteins which contain two EF-hand calcium binding sites. S100A6 may function as a calcium sensor to activate several processes in the calcium signal transduction pathway of cell growth, proliferation, secretion and exocytosis. Although S100A6 is a cytoplasmic protein, it can be located in the nuclear and cytoplasmic membranes in the presence of Ca2+ (PMID: 37670392).

Publications

Species	Application	Title
human	WB	Open Med (Wars); S100A6 promotes proliferation and migration of HepG2 cells via increased ubiquitin-dependent degradation of p53.; Authors - Dongqiang Song