CoraLite® Plus 647-conjugated FSP1/S100A4 Monoclonal antibody

• Featured Product
• KD/KO Validated

FSP1/S100A4 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2G11B4

Cat no : CL647-66489

Synonyms

S100A4, Fibroblast-specific protein-1, Fibroblast-specific protein 1, Fibroblast Specific Protein, CAPL

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Validation Data Gallery

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  IF Staining of HeLa using CL647-66489

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 S100A4 antibody (CL647-66489, Clone: 2G11B4 ) at dilution of 1:200.

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  FC experiment of HeLa using CL647-66489

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647-conjugated S100A4 Monoclonal antibody (CL647-66489, Clone:2G11B4)(red), or 0.4 ug CoraLite® Plus 647 Mouse IgG2a isotype control (11A1B2) (CL647-66360-2, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66489 targets FSP1/S100A4 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FSP1/S100A4 fusion protein Ag9019
• Full Name: S100 calcium binding protein A4
• Calculated Molecular Weight: 101 aa, 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC016300
• Gene Symbol: S100A4
• Gene ID (NCBI): 6275
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

S100A4 is a member of the S100 family of calcium-binding proteins. The S100 family members have been involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A4 is known to localize to and function in the nucleus, cytoplasm of cells, and the extracellular space. S100A4 has also been shown to be associated with tumor growth, motility, invasion, metastasis, angiogenesis, apoptosis, and chemoresistance. It is a fibroblast-specific protein associated with mesenchymal cell morphology and motility, is expressed during epithelial-mesenchymal transformations (EMT) in vivo (PMID: 9362334). It is a specific prognostic marker for renal survival in patients with IgAN (PMID: 16105038). It is also an improved marker for lung fibroblasts that could be useful for investigating the pathogenesis of pulmonary fibrosis(PMID: 15618458). Overexpression of S100A4 is correlated with a worse prognosis inpatients with various types of cancer.