S100A14 Recombinant antibody, PBS Only

S100A14 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240528B6

Cat no : 83559-5-PBS

Synonyms

S114, S100A15, S100 calcium-binding protein A14, Protein S100-A14, Protein S100 A14

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Validation Data Gallery

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  WB analysis using 83559-5-RR (same clone as 83559-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83559-5-RR (S100A14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83559-5-RR (same clone as 83559-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83559-5-RR (S100A14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83559-5-RR (same clone as 83559-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 83559-5-RR (same clone as 83559-5-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug S100A14 Recombinant antibody (83559-5-RR, Clone:240528B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83559-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83559-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83559-5-RR against Human S100A14 were performed. The affinity constant is 4.90 nM.

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Product Information

The immunogen of 83559-5-PBS is S100A14 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: S100A14 fusion protein Ag0757
• Full Name: S100 calcium binding protein A14
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC005019
• Gene Symbol: S100A14
• Gene ID (NCBI): 57402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The S100 family is a multifunctional group of EF-hand type calciumbinding proteins. S100A14 protein (previously known as BCMP84, S100A15) is a recently identified member of the S100 family. It is differentially expressed in a number of different human malignancies like ovary, breast, uterus, kidney, rectum and colon cancers, and esophageal and oral squamous cell carcinomas (OSCCs).7